Coding
Part:BBa_K2074032:Design
Designed by: Ruicheng Dai Group: iGEM16_FAFU-CHINA (2016-10-09)
Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2195
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 517
Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 2029
Illegal NgoMIV site found at 2329 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 49
Illegal BsaI.rc site found at 244
Illegal BsaI.rc site found at 1459
Illegal SapI.rc site found at 1440
Design Notes
To identify there is no restriction enzyme cutting site which we choosed.
Source
DNA from a synthesis company.