Coding

Part:BBa_K2074032:Design

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)


Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
    Illegal NgoMIV site found at 2329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal SapI.rc site found at 1440


Design Notes

To identify there is no restriction enzyme cutting site which we choosed.


Source

DNA from a synthesis company.

References